FastQCFastQC Report
Mon 27 Feb 2023
SRR3128192.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128192.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880336
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222021.1807464197888038No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36600.19464606325677963No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA34510.18353102849703457No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC33680.1791169237838344TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG33490.178106466078403TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG31700.16858689085354958TruSeq Adapter, Index 13 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAGCCACTT30670.16310914645042163No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC19440.10338577786097804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70350.084.14491
ACGGGTA1900.074.2054444
CCGTACA14450.073.828853
GAATAGG10600.073.6908341
ATAGGGC10850.073.202363
CGTACAC14600.073.070344
CGTAGGG5450.072.43542
ATAGGGA17150.070.70073
AGTACGG1800.070.583391
TCCGTAC15900.069.756232
ATCCGTA16100.068.975911
AGGGTAC9050.068.54785
AGTAAGG11950.068.516091
GGTAAGG11700.068.3713761
AGTAGGG32450.068.06932
AGGGATA17300.067.371115
GTACGGG3150.067.138262
AATAGGG27450.066.7713852
ATAGAGG13100.066.452561
GATAGGG27650.066.2884142
Produced by FastQC (version 0.11.5)