FastQCFastQC Report
Mon 27 Feb 2023
SRR3128191.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128191.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2081870
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264871.272269642196679No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT41510.19938805016643688No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG38100.18300854520214999TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC36840.17695629410097652TruSeq Adapter, Index 16 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG35060.16840628857709655TruSeq Adapter, Index 13 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24290.1166739517837329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87150.084.551531
ACGGGTA1950.077.134284
GAATAGG13800.074.625091
CGTAGGG5750.073.572782
ATAGAGG14650.071.2582551
TAGGGTA6150.071.0787664
AGTAAGG11100.070.747861
ATAAGGG26600.070.1538242
TAGGGCA9550.068.9059454
TAAGGGA15100.068.795143
AGGGATG30450.068.379775
ATAGGGA18500.068.3475653
AGTAGGG37200.068.232822
ATAGGGC12650.067.999083
ATAGCGG5750.067.878171
AGGGCAT17500.067.41355
AAGAGGG70800.067.18772
GTACGGG3650.066.965782
TAGAGGG41150.066.937622
TAGACGG3600.066.617371
Produced by FastQC (version 0.11.5)