FastQCFastQC Report
Mon 27 Feb 2023
SRR3128190.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128190.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815885
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235581.2973288506706098No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT36090.19874606596783387No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC30770.1694490565206497TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG30220.1664202303559972TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTG29300.16135383022603303TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA156.903603E-493.997475
CGTTTTT73000.085.3543551
GAATAGG11650.077.5596541
TAGGGCA8300.075.388034
ACGGGTA1550.072.846454
CGTAGGG5600.071.410122
TAGGGTA5150.071.255154
ATAGGGA17250.070.092713
AGGGATG25100.069.8427355
AGTAGGG31700.069.3084952
GTAGGGA14900.069.149133
TAGAGGG33850.068.936912
AGTAAGG10800.068.8484041
GGTAAGG10350.068.658951
ATAGGGC9900.068.431523
ATAGAGG11900.068.416321
TATAGGG15300.067.956292
AGGGTAC9200.067.432965
AAGAGGG65150.066.36362
TAGGGAC10200.065.957584
Produced by FastQC (version 0.11.5)