FastQCFastQC Report
Mon 27 Feb 2023
SRR3128189.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128189.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619110
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356532.2020122165881255No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG33070.204248012797154No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA32680.20183928207472007No Hit
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC27920.17244041479578287Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT26990.1666965184576712No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22670.1400151935322492No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTG21380.13204785345035236No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTT20640.12747744131034952No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19790.12222764358196787No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAAACAGT16310.10073435405871126No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110000.087.735111
GGTAAGG10300.077.205791
CCGTACA10550.076.621923
TAGGGCA7800.075.919434
TCCGTAC10850.074.505642
ATAGGGC9400.074.496433
GAATAGG8200.074.024631
TAGGGTA5000.073.316484
AGGGATG25950.071.71915
TATAGGG12200.071.654522
TAAGGGA14500.071.306923
GTAGGGT5700.070.908883
ATCCGTA11600.070.581631
ATAGGGA14350.070.0872343
CGTAGGG5300.070.055412
AGTAGGG27000.069.454372
CGTACAC11450.069.367854
GTAGGGC6750.068.930023
GAGGGAT18950.068.6985554
AGGGCAT14400.068.2120065