FastQCFastQC Report
Mon 27 Feb 2023
SRR3128186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526127
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163911.0740259493475968No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAA40300.26406714513274454No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA34590.22665217246009015No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG29760.1950034302518729No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC27240.178491043012803No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG26340.17259376185599232No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19910.13046096425788942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60900.085.60871
ATAGCGG3050.078.680261
CGTAGGG4200.076.090122
AGGGATG28200.075.494935
TAGGGCA10450.073.7558064
CCGTACA18500.073.162653
CGTACAC18500.072.654584
TAGCGGG7900.072.57742
TCCGTAC19100.072.340682
AGGGCAT15450.071.7880555
AGGGTAC7100.071.488155
GGTAAGG9950.071.40841
AGTAGGG26250.070.5400162
GGATGGC26450.069.1182257
GTAGGGA13600.069.1133
ATCCGTA20050.068.9967041
AAGGGAT28600.068.852014
AAGGGCA26100.068.604584
GTAAAGG10800.068.40241
AGAGGGC21750.068.2804643
Produced by FastQC (version 0.11.5)