FastQCFastQC Report
Mon 27 Feb 2023
SRR3128185.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128185.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705230
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194251.139142520363822No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG34110.20003166728241936No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC30800.18062079602165104No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG29560.17334904968831186No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24670.14467256616409518No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAACGGAA23700.138984183951725No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23490.13775267852430464No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72550.085.03341
TAGGGCA11100.078.7575154
AGGGATG35500.074.538755
AGGGCAT17050.073.050565
GTACGGG4000.072.850712
GTAGGGT6200.072.77493
CGTAGGG4400.072.637072
AGTAGGG28550.071.776532
TGTAGGG12950.069.684072
AAGGGTA14750.069.146424
GGATGGC30200.069.1000067
TAAGGGA15900.068.874883
TATAGGG13450.068.840822
CGAAGGG18250.068.762312
AAGGGAT33450.068.709184
ATAAGGG23200.068.677392
TAGGGCG3500.068.4863744
AGAGGGC25200.068.449073
GAATAGG11700.068.321311
ACGGGTA2000.068.1506654
Produced by FastQC (version 0.11.5)