FastQCFastQC Report
Mon 27 Feb 2023
SRR3128184.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128184.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462164
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167791.1475456925488523No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG27060.1850681592488941No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC24850.16995357565909158No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG22970.15709592084061705No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19130.13083347695607334No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62900.086.562711
ATAGCGG2850.080.979461
AGGGATG30750.078.4211965
GAGGGTA9600.075.536024
TAGGGCA10650.074.2786944
GTAGGGC8900.074.070053
CGTAGGG3150.073.247052
GTACGGG2700.073.247042
TAAGGGA14950.072.127173
ACGGGTA1900.071.8702244
GAGGGAT26500.071.786054
AGTAGGG26250.071.7522052
AAGGGAT29950.071.220664
TAGAGGG25600.071.182892
TATAGGG11400.071.044132
TAGGGTA5250.070.8553164
AGGGTAG6950.070.34125
GTAGGGT4850.069.902923
AGGGCAT14750.069.474545
TACGGGT950.069.3919453
Produced by FastQC (version 0.11.5)