FastQCFastQC Report
Mon 27 Feb 2023
SRR3128183.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128183.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241339
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371621.6580267420501762No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG33970.1515611873081225No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC29150.13005618516431472TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT28120.12546071790121888No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA28000.1249253236569747No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCAAAGGTCC25980.11591285387886438No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT22820.10181413878043437No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT123300.086.6730351
TAGGGCA13150.072.907944
ATAGGGA19200.072.2089843
ATAGGGC13450.071.2817463
CGTACAC15000.070.1822364
CCGTACA15150.070.107793
GGTAAGG14250.068.9874651
ATCCGTA16100.068.3642041
TCCGTAC15750.068.332192
TATAGGG18250.068.242262
GAATAGG14000.068.2035141
AGGGATG32450.068.0696645
CGAAGGG22250.067.591242
ATAGCGG4350.067.041111
ATAGAGG17850.066.932071
TAGAGGG45000.066.840222
ATGCGGG11750.066.7957842
AGGGTAC9450.066.64135
TAAGGGA17450.065.4457243
CGTAGGG5400.065.273652
Produced by FastQC (version 0.11.5)