FastQCFastQC Report
Mon 27 Feb 2023
SRR3128182.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128182.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2498834
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT451171.8055220955053435No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG38200.15287129917393474No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC31560.12629890580966963TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT29810.11929563948625639No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT141100.087.071011
GTACGGG6150.074.13572
ATAGGGC15300.070.9661563
ATAACGG2200.070.526511
GTAGGGC11950.070.4070053
AGGGATG36350.070.3437045
ATGCGGG11300.070.2973562
TAGGGCA13450.070.243244
TATAGGG20700.069.029392
ACGGGAT6950.068.983644
ATAGGGA23400.068.496653
AGTAGGG40900.067.1151052
GAATAGG14100.067.025191
TAGCGGG13150.066.841572
CGAAGGG23150.066.39382
ATAGAGG18750.066.200891
GGATAGG16100.065.999971
TAGAGGG45850.065.815242
GGTAAGG17800.065.771911
CGTAGGG8200.065.346422
Produced by FastQC (version 0.11.5)