FastQCFastQC Report
Mon 27 Feb 2023
SRR3128181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162729
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399391.846694615922753No Hit
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTG30180.13954591629371965No Hit
CGCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCT27330.12636812101747377No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGC26240.12132819229778674TruSeq Adapter, Index 22 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22660.1047750319156954No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTT21680.1002437198557933No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129550.086.255591
AGTACGG3550.072.9253161
ACGGGAT5850.071.5808944
TAGGGCA12950.071.2113654
CGAAGGG19950.070.280752
AGGGATG31200.070.053425
ATAGGGA21350.068.977823
GAATAGG11700.068.794581
TAGACGG3100.068.3273851
GGTAAGG13250.068.207061
TAGAGGG43900.068.1640852
TAGGGCG4150.068.024634
AGTAGGG35850.067.9835052
GTACGGG6300.067.961642
TAAGGGA16850.067.853053
ATAGGGC13500.067.613113
ATAGCGG4050.067.408851
TAGGGTA6250.066.999724
TATAGGG17850.066.951222
AGGGTAC11100.066.4825445
Produced by FastQC (version 0.11.5)