FastQCFastQC Report
Mon 27 Feb 2023
SRR3128180.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128180.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040094
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272701.3367031126997089No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA38690.18964812405702874No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTGTAAC37450.18356997275615733No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG37200.18234453902614292Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC36490.178864307232902Illumina PCR Primer Index 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG32000.15685551744184337Illumina PCR Primer Index 2 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22510.1103380530504967No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21320.10450498849562814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT88300.086.157011
TCCGTAC20900.079.603052
CCGTACA20350.078.2903063
ATCCGTA21850.077.085541
CGTACAC20650.076.470154
ATAGGGA19550.074.762933
GAATAGG11700.073.587931
AGGGATG27750.073.3326955
GTAGGGT6750.072.410623
ATAGGGC9550.071.849243
AGATAGG13250.070.305721
GAGGGAT25200.069.376944
ATAGCGG4750.069.333921
AGTAGGG33150.069.042762
GTAGGGA14650.068.9721153
TAGGGTA6900.068.793124
TAGCGGG11900.068.323782
AGTAAGG12700.067.79361
ATAAGGG27250.066.744762
GTACGGG4100.066.483962