Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128180.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 2040094 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 47 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 27270 | 1.3367031126997089 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 3869 | 0.18964812405702874 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTGTAAC | 3745 | 0.18356997275615733 | No Hit |
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG | 3720 | 0.18234453902614292 | Illumina PCR Primer Index 2 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC | 3649 | 0.178864307232902 | Illumina PCR Primer Index 2 (95% over 24bp) |
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG | 3200 | 0.15685551744184337 | Illumina PCR Primer Index 2 (95% over 23bp) |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 2251 | 0.1103380530504967 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2132 | 0.10450498849562814 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 8830 | 0.0 | 86.15701 | 1 |
TCCGTAC | 2090 | 0.0 | 79.60305 | 2 |
CCGTACA | 2035 | 0.0 | 78.290306 | 3 |
ATCCGTA | 2185 | 0.0 | 77.08554 | 1 |
CGTACAC | 2065 | 0.0 | 76.47015 | 4 |
ATAGGGA | 1955 | 0.0 | 74.76293 | 3 |
GAATAGG | 1170 | 0.0 | 73.58793 | 1 |
AGGGATG | 2775 | 0.0 | 73.332695 | 5 |
GTAGGGT | 675 | 0.0 | 72.41062 | 3 |
ATAGGGC | 955 | 0.0 | 71.84924 | 3 |
AGATAGG | 1325 | 0.0 | 70.30572 | 1 |
GAGGGAT | 2520 | 0.0 | 69.37694 | 4 |
ATAGCGG | 475 | 0.0 | 69.33392 | 1 |
AGTAGGG | 3315 | 0.0 | 69.04276 | 2 |
GTAGGGA | 1465 | 0.0 | 68.972115 | 3 |
TAGGGTA | 690 | 0.0 | 68.79312 | 4 |
TAGCGGG | 1190 | 0.0 | 68.32378 | 2 |
AGTAAGG | 1270 | 0.0 | 67.7936 | 1 |
ATAAGGG | 2725 | 0.0 | 66.74476 | 2 |
GTACGGG | 410 | 0.0 | 66.48396 | 2 |