FastQCFastQC Report
Mon 27 Feb 2023
SRR3128178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976992
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282431.4285844353441997No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG33130.1675778151858986Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC31830.16100216895161942Illumina PCR Primer Index 2 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG27980.14152813971933118Illumina PCR Primer Index 2 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22770.11517497288810476No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20640.10440102944270892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90950.086.245591
TAGGGCA9100.077.0223854
GAATAGG13150.071.566251
AGTAGGG33050.070.31172
GAGGGAT24050.069.63174
AGGGCAT14900.068.766935
ACGGGAT6100.068.632884
AGGGATG28450.068.560715
TAGAGGG35450.068.338132
GTAGGGC7400.068.018043
ATAGGGC10450.067.972423
GTACGGG4500.067.947412
GGTAAGG12300.067.713021
TGTAGGG15000.067.424742
AAGAGGG68350.067.308882
AGTAAGG12750.067.1684341
ATAGGGA16700.067.039773
GTAGGGA14250.067.01213
AGGGTAC9650.066.2401055
ATAAGGG26150.065.838732
Produced by FastQC (version 0.11.5)