FastQCFastQC Report
Mon 27 Feb 2023
SRR3128177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391875
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166671.197449483610238No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA54580.39213291423439606No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCAGTGTG54040.3882532555006736No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG44060.3165514144589133No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC42940.3085047148630444TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG37330.2681993713515941No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18800.13506960035922766No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATG17860.12831612034126627No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14290.10266726537943421No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTCAGTGTGTC14010.10065559048046699No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT58750.085.868491
TAGGGCA7100.076.121764
CGGGTAT1250.075.195055
CCGTACA25700.074.975623
AGGGTAC5750.074.377725
TCCGTAC27250.074.3327262
ATAACGG1400.073.9505841
ATCCGTA27650.073.695281
ATAGGGC8850.073.283323
ATAGGGA11500.073.151723
CGTACAC26200.072.827274
AGGGATG23100.072.2246865
GCATAGG3100.069.830171
AGTAGGG20150.069.737352
CGTAGGG3950.069.0081252
GGTAAGG8200.068.867511
AAGGGAT27450.068.654874
ACGACCA4750.068.2691967
CGAAGGG12550.068.1548842
TATAGGG9350.067.85652