FastQCFastQC Report
Mon 27 Feb 2023
SRR3128176.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128176.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535459
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197461.2859998215517314No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG48960.3188623076226718No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC46520.30297129392579025TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG40850.2660442252121353No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA37070.24142617940303193No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCAGTGTG30180.19655360384093615No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22720.14796878327588037No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16610.10817612192836148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73850.086.28091
ATAGCGG2850.075.899481
ATAGGGA11800.073.684323
AGGGAAT17550.072.305615
AGGGATG25150.071.1987465
GCATAGG4700.071.0372851
TAAGGGA12350.070.783393
TAGCGGG7450.070.024832
TAGAGGG29850.069.907542
TAGGGCA8200.069.351664
CGGGTAT1850.068.592625
CCGTACA18000.068.409153
AGGGCAT15200.068.333565
GTAGGGC6400.068.294913
GAGGGAT19350.068.251274
AGGGTAC6000.068.148045
CGAAGGG14000.068.148042
TCCGTAC18700.068.110342
AGAGGGC20450.068.027383
AAGGGAT30250.067.7402044
Produced by FastQC (version 0.11.5)