FastQCFastQC Report
Mon 27 Feb 2023
SRR3128175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344679
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173881.2930967167628853No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG41100.30564915492842526No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC39010.2901064120135735TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG33480.24898135540154936No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20430.15193217117245084No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA18510.1376536705042616No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCAGTGTG16090.11965681028706478No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14850.11043527860552593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61300.087.174761
ATAGCGG2250.079.516351
CGTAGGG3150.074.716712
GCATAGG4250.074.223461
TAGGGCA7500.074.0592044
ATAGGGA11950.074.053953
AGGGATG22800.072.1460655
TAGAGGG22150.071.19172
AGTAGGG19550.069.824772
GAGGGAT17250.069.5844
GTAGGGA10500.069.486543
ATAACGG950.069.384061
ATAGGGC10250.069.34443
GTAGGGC6550.068.9903263
TATAGGG10250.068.885162
TAAGGGA10600.068.831013
ATGCGGG6550.068.2716752
AAGGGAT26850.067.495484
AGATAGG9700.067.468091
ATAAGGG20200.067.34492
Produced by FastQC (version 0.11.5)