FastQCFastQC Report
Mon 27 Feb 2023
SRR3128174.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128174.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874536
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231131.2329984593520744No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39420.21029204027023218No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA38050.2029835649995519No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAGGACTC31110.16596106983274794No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC28020.14947699057260036TruSeq Adapter, Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG27720.14787659452792584TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG23390.12477754494979025TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79300.087.7053761
AGGGATG39300.078.9259645
TCCGTAC18050.077.85072
CCGTACA17950.076.189843
AGGGTAC10300.075.742465
ATAGCGG3900.074.8086851
CGTAGGG5850.074.7129062
CGTACAC18100.074.519834
GGATGGC32950.073.882727
AGGGCAT19750.073.05335
TAGCGGG9200.072.538582
AAGGGTA17000.072.43044
CGAAGGG19900.072.266472
GAGGGAT33300.071.694864
TAGGGCA10750.071.26034
ATAGGGA20650.070.552143
ATCCGTA20100.070.468561
TACGGGT2900.069.684953
ACGGGTA2300.069.473574
AAGGGAT38050.069.41454