Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128174.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1874536 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 23113 | 1.2329984593520744 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 3942 | 0.21029204027023218 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 3805 | 0.2029835649995519 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAGGACTC | 3111 | 0.16596106983274794 | No Hit |
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC | 2802 | 0.14947699057260036 | TruSeq Adapter, Index 8 (95% over 23bp) |
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG | 2772 | 0.14787659452792584 | TruSeq Adapter, Index 13 (95% over 22bp) |
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG | 2339 | 0.12477754494979025 | TruSeq Adapter, Index 13 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 7930 | 0.0 | 87.705376 | 1 |
AGGGATG | 3930 | 0.0 | 78.925964 | 5 |
TCCGTAC | 1805 | 0.0 | 77.8507 | 2 |
CCGTACA | 1795 | 0.0 | 76.18984 | 3 |
AGGGTAC | 1030 | 0.0 | 75.74246 | 5 |
ATAGCGG | 390 | 0.0 | 74.808685 | 1 |
CGTAGGG | 585 | 0.0 | 74.712906 | 2 |
CGTACAC | 1810 | 0.0 | 74.51983 | 4 |
GGATGGC | 3295 | 0.0 | 73.88272 | 7 |
AGGGCAT | 1975 | 0.0 | 73.0533 | 5 |
TAGCGGG | 920 | 0.0 | 72.53858 | 2 |
AAGGGTA | 1700 | 0.0 | 72.4304 | 4 |
CGAAGGG | 1990 | 0.0 | 72.26647 | 2 |
GAGGGAT | 3330 | 0.0 | 71.69486 | 4 |
TAGGGCA | 1075 | 0.0 | 71.2603 | 4 |
ATAGGGA | 2065 | 0.0 | 70.55214 | 3 |
ATCCGTA | 2010 | 0.0 | 70.46856 | 1 |
TACGGGT | 290 | 0.0 | 69.68495 | 3 |
ACGGGTA | 230 | 0.0 | 69.47357 | 4 |
AAGGGAT | 3805 | 0.0 | 69.4145 | 4 |