FastQCFastQC Report
Mon 27 Feb 2023
SRR3128173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2090706
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274441.3126666303153096No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45380.21705586533926818No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG31230.14937537846067309TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC29660.1418659534147795TruSeq Adapter, Index 8 (95% over 23bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA25990.12431207448584354No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG24870.11895503241488761TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94950.087.767081
ACGGGTA2700.078.332414
AGGGATG44700.075.91415
CGTAGGG5950.075.831052
AAGGGTA17750.073.345624
CGAAGGG23000.071.929592
GGATGGC38150.071.209267
GAGGGAT37100.071.195934
ATGCGGG11050.071.030842
AGGGTAC10700.070.71885
TAGGGCA12750.070.03844
CGGGATG8600.069.406165
GAATAGG14350.069.150211
TAAGCGG5650.069.08641
AAGGGAT42800.069.0716254
AGTAAGG13450.068.882181
AGGGCAT22200.068.80555
AGAGGGC31050.068.5692443
ATAGCGG4550.068.2174451
GTAAGGG33450.068.145692
Produced by FastQC (version 0.11.5)