FastQCFastQC Report
Mon 27 Feb 2023
SRR3128172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816045
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240821.3260684619599183No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36860.20296853877519555No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG26850.14784875925431365TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC25080.13810230473363821TruSeq Adapter, Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG21960.12092211371414255TruSeq Adapter, Index 13 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86000.087.761551
CGTAGGG4950.082.685362
ATAGCGG3450.080.46951
AGGGATG36650.077.3241655
AAGGGAT34150.071.910844
ACGGGTA2750.071.850724
TAGGGCA9950.071.39514
GGATGGC30850.071.295587
GAGGGAT30850.070.91084
AAGGGTA15850.070.641884
ATAGGGC11350.070.464053
CGGGATG7800.069.8932655
GAATAGG11800.069.783761
CGAAGGG19250.069.162442
AGTAAGG10850.068.9551
TAGCGGG9700.068.870172
GTAGGGC8850.068.574243
AGTAGGG30650.068.457172
ATAACGG1100.068.442471
AGAGGGC26650.068.3169253
Produced by FastQC (version 0.11.5)