FastQCFastQC Report
Mon 27 Feb 2023
SRR3128171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1377890
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167291.214102722278266No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTTGAAGG100080.7263279361922941No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA45160.32774749798605113No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC41730.302854364281619Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG41290.2996610759930038No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG36960.2682362162436769No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATG22420.16271255325171094No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCTTGAAGGTC21000.1524069410475437No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62300.086.7805251
ATAGCGG2250.079.4678341
CCGTACA36200.076.981163
CGTACAC36250.076.22684
TAGGGCA6850.076.150154
ATCCGTA38600.075.6997761
TCCGTAC38250.075.435422
CGTAGGG4400.072.626412
GTAGGGC5500.071.771983
AGGGCAT11550.071.202365
TACACCC37250.070.774196
GTACACC38900.070.671555
ATAGGGA12400.070.111363
TAGGGTA5950.069.503084
GTACGGG3250.069.405872
GCATAGG3800.069.3417361
AGGGTAC7750.069.126015
GTAGGGT5850.069.084553
ATGAGGG19500.068.44192
ATAGAGG8800.068.441191
Produced by FastQC (version 0.11.5)