FastQCFastQC Report
Mon 27 Feb 2023
SRR3128170.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128170.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530425
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201321.31545159024454No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTTGAAGG57990.378914353855955No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC46180.3017462469575445Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG45900.2999166898083866No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG41600.27181991930346144No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA31580.20634790989431043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74100.086.068741
TAGGGCA8450.077.315034
CGTAGGG3950.076.1533052
ATAGCGG2950.074.934151
ACGGGAT5100.073.726854
GTAGGGC6550.073.1921843
TAGACGG1750.072.5654141
GGATAGG12450.072.5330351
GTAGGGT6500.071.585933
GTAGGGA12500.071.065313
GAATAGG7500.070.2361451
TAGGGTA6300.070.128284
AGGGTAA7850.070.0522465
AGTAGGG22950.069.835712
CGAAGGG13850.069.2287142
ATAGAGG10250.068.828991
ATAGGGA13950.068.732453
TAGGGAC8600.068.315214
AGGGATG20400.067.966945
TGTAGGG10600.067.840872
Produced by FastQC (version 0.11.5)