FastQCFastQC Report
Mon 27 Feb 2023
SRR3128169.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128169.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315134
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172601.31241379205465No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC38980.2963956524582286Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG36460.27723410694271455No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG33200.2524457583789941No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCTTGAAGG30630.23290402346833097No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA16080.12226890947994652No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13380.10173868214189581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62850.086.738091
ATAGCGG3250.082.56541
GTAGGGC5050.078.279073
TAGGGCA7600.078.021574
GAATAGG7250.075.3228151
CGTAGGG3950.073.867322
AGGGTAA6650.072.089915
AGTAGGG20550.071.449712
TAGGGTA5750.070.386334
AAGGGTA8600.070.043734
AGGGTAC7100.069.506735
GAGGGAT15000.069.3364
TAGCGGG7550.069.188472
TAGAGGG20250.069.0222552
TAGACGG1450.068.180141
CGAGGGA5800.068.156783
GTAGGGT5750.067.9313
AGGGAAT15650.067.87195
CGAAGGG12000.067.453572
AGGGCAT11000.067.081525