FastQCFastQC Report
Mon 27 Feb 2023
SRR3128167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048564
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255941.2493629684012801No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC60880.2971837833721573No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG60640.29601223100669544No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG54380.2654542401408987No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGTCAAAG53180.25959647831358945No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA37490.18300624242152064No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22210.10841740848711585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98450.086.399231
CGTAGGG6550.081.085632
CGAAGGG19350.079.670892
TAGGGCA12950.076.215924
TAGCGGG9400.073.501512
CGAGGGA10150.073.164063
GAATAGG10800.072.707591
ATAGCGG3300.072.6680151
ATAGGGC12550.072.280373
GTAGGGT8650.070.637293
TACGGGT2000.070.501443
TAGGGTA7100.070.168744
AGGGCAT18750.069.43445
AAGGGCG7200.069.1941764
ATGCGGG9450.069.13372
AGGGTAT11700.068.692045
TAGGGCG3150.068.634654
AGTAGGG32450.068.3650362
ATAGGGA22400.068.193373
AAGGGAC19250.067.875054
Produced by FastQC (version 0.11.5)