FastQCFastQC Report
Mon 27 Feb 2023
SRR3128166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795503
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221271.2323566153885568No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG47600.26510676952363765No Hit
CTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC46930.26137522465849405No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTG45720.25463616602144357No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGTCAAAG27570.15355028646568677No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC18940.10548576081465752No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA18870.10509589791829921No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86750.087.251721
CGAAGGG16300.079.972482
TAGGGCA10500.075.7435764
CGTAGGG5550.072.92122
TAGCGGG6650.070.766342
AAGGGCG7000.070.5894244
ACGGGAT6050.070.006044
TAAGGGA15250.069.1236343
TAGAGGG28600.068.943982
ACGGGTA2050.068.867734
TACGGGT1300.068.7794343
ATAGGGA18400.068.543363
AGAGGGC21900.068.333143
ATAGGGC10300.068.076533
ATGCGGG7200.067.9752
AGGGATG23900.067.460325
CGAGGGA9800.067.228023
GGTAAGG9950.066.704241
GAATAGG9500.066.395491
AAGCGGG17300.066.37312
Produced by FastQC (version 0.11.5)