Basic Statistics
Measure | Value |
---|---|
Filename | SRR3128165.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1738065 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 22700 | 1.3060501189541243 | No Hit |
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA | 5262 | 0.30275047250822035 | No Hit |
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCTGTG | 4664 | 0.2683443944846712 | No Hit |
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG | 4026 | 0.23163690655988123 | No Hit |
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC | 3995 | 0.2298533138864197 | TruSeq Adapter, Index 15 (95% over 21bp) |
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG | 3229 | 0.18578131427765934 | No Hit |
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 2493 | 0.1434353720948296 | No Hit |
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT | 1902 | 0.10943204080399754 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTTTTT | 7805 | 0.0 | 85.05432 | 1 |
CCGTACA | 2500 | 0.0 | 78.39047 | 3 |
ACGGGTA | 145 | 0.0 | 77.78763 | 4 |
TCCGTAC | 2630 | 0.0 | 77.553474 | 2 |
CGTACAC | 2535 | 0.0 | 77.30817 | 4 |
AGTAGGG | 2790 | 0.0 | 74.62199 | 2 |
ATCCGTA | 2755 | 0.0 | 74.45728 | 1 |
ATAGCGG | 320 | 0.0 | 73.512695 | 1 |
GGTAAGG | 1110 | 0.0 | 73.32726 | 1 |
AGGGAAT | 1850 | 0.0 | 72.90838 | 5 |
AGTAAGG | 1125 | 0.0 | 72.34956 | 1 |
AGGGATG | 2725 | 0.0 | 71.91787 | 5 |
TAGGGCA | 1110 | 0.0 | 70.70673 | 4 |
GTAGGGA | 1450 | 0.0 | 70.33298 | 3 |
TAAGGGA | 1350 | 0.0 | 69.972855 | 3 |
AGGGTAC | 945 | 0.0 | 69.12741 | 5 |
TAGAGGG | 3305 | 0.0 | 68.68201 | 2 |
AAGGGAT | 2845 | 0.0 | 68.05848 | 4 |
GAATAGG | 975 | 0.0 | 68.038826 | 1 |
AAGGGTA | 1370 | 0.0 | 67.922226 | 4 |