FastQCFastQC Report
Mon 27 Feb 2023
SRR3128165.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128165.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738065
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227001.3060501189541243No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA52620.30275047250822035No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATGCTGTG46640.2683443944846712No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG40260.23163690655988123No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC39950.2298533138864197TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG32290.18578131427765934No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24930.1434353720948296No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19020.10943204080399754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78050.085.054321
CCGTACA25000.078.390473
ACGGGTA1450.077.787634
TCCGTAC26300.077.5534742
CGTACAC25350.077.308174
AGTAGGG27900.074.621992
ATCCGTA27550.074.457281
ATAGCGG3200.073.5126951
GGTAAGG11100.073.327261
AGGGAAT18500.072.908385
AGTAAGG11250.072.349561
AGGGATG27250.071.917875
TAGGGCA11100.070.706734
GTAGGGA14500.070.332983
TAAGGGA13500.069.9728553
AGGGTAC9450.069.127415
TAGAGGG33050.068.682012
AAGGGAT28450.068.058484
GAATAGG9750.068.0388261
AAGGGTA13700.067.9222264