FastQCFastQC Report
Mon 27 Feb 2023
SRR3128163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1692441
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240761.4225606682891752No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC37380.22086442008909024TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG37230.21997812626850805No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG29340.17335907130588304No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC24250.1432841676607929No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19070.11267748772335344No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA17560.10375546326282571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81900.086.650241
ACGGGTA1800.081.033464
CGTAGGG4900.079.69982
GGTAAGG11450.073.569841
TAGGGCA8450.072.944044
TAGGGTC4250.070.854314
GAATAGG11650.070.691041
AGTAGGG28450.070.45352
CGAGGGA10500.070.353483
TATAGGG13250.069.956092
AGGGATG27600.069.4765855
AGTAAGG10650.069.374891
TAGAGGG31200.069.371082
ACGGGTC1900.069.339334
GTAGGGT7400.068.670043
ATAGAGG10300.068.5340351
TAGGGTA6300.067.963554
AGGGACT22050.067.780685
AGGGAAT19600.067.620815
AAGGGAT30000.067.440754
Produced by FastQC (version 0.11.5)