FastQCFastQC Report
Mon 27 Feb 2023
SRR3128162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250848
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345481.534888184364293No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACCCGTAA39520.17557827094499495No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG38880.17273489813616913No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA38680.17184634413341104No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC35860.15931773269452226No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG28230.1254193974893018No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT23820.10582678172848634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110250.087.3920751
CCGTACA21100.076.618443
CGTACAC21200.075.813684
GTACGGG4100.074.505232
TCCGTAC21950.074.293752
CGTAGGG6200.074.2833942
ATCCGTA22400.073.5090261
ATAGGGC14500.072.600123
CGAAGGG22700.071.010992
TAGGGCA15150.070.415864
TAGGGTA7350.069.6941764
ATAGCGG4550.069.27621
ATAGGGA17450.067.5982743
AGGGCAT22100.066.9846955
AGTAAGG15250.066.943831
AAGGGCA35550.066.891084
GGTAAGG12250.066.824211
AGAGGGC35050.066.772663
AGTACGG3050.066.326831
ATGCGGG12200.065.8708952
Produced by FastQC (version 0.11.5)