FastQCFastQC Report
Mon 27 Feb 2023
SRR3128161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492168
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404801.624288571235968No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG42220.16941072993473955No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC41720.1674044446441813No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG31950.12820163006667287No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA27730.11126858221436114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT131850.086.802671
CGTAGGG7000.075.2035452
TAGGGCA16400.075.088914
ATAGCGG5250.071.651181
TAGGGTA7750.070.958184
ATAGGGC16200.069.632913
AGGGCAT26200.069.4255455
TAGAGGG45350.069.337332
GTAGGGC10900.068.994083
CGAAGGG28750.068.664112
ATAGGGA20450.068.4922263
AGTAGGG42650.068.326782
TATAGGG19750.067.825982
TAGGGCG4700.067.003164
GGTAAGG14950.066.9932561
CCGTACA14250.066.957543
AGGGTAC12750.066.723375
TAGCGGG12900.066.31322
TAAGGGA23000.066.2118153
AGAGGGC39800.066.133773
Produced by FastQC (version 0.11.5)