FastQCFastQC Report
Mon 27 Feb 2023
SRR3128160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186417
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357141.6334486971149604No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG34550.15802109112763027No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC32850.15024581312713906No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG25440.1163547484308803No Hit
CGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT22190.10149024637111767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT114500.087.214411
ACGGGTA2550.071.967894
ATAGCGG3800.071.8616941
CGTAGGG5650.071.6249542
GGTAAGG13350.070.534531
AGGGTAC11100.069.8648155
ATAGAGG15550.069.638671
ATAGGGA18800.069.082113
CGAAGGG21550.068.782442
TAGGGTA7150.068.444994
TAGAGGG43400.068.090152
GTAGGGA15200.067.178533
TAGGGCA12150.067.001444
AAGGGTA14400.066.989344
AGAGGGC33800.066.546553
TAGCGGG12050.066.385962
TAAGGGA17600.066.038723
AGTAGGG37300.065.726912
AGGGCAT19600.065.704045
ATAGGGC13550.065.635213
Produced by FastQC (version 0.11.5)