FastQCFastQC Report
Mon 27 Feb 2023
SRR3128159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256678
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136621.0871519991596892No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGACATC114140.9082676707955419No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG59170.4708445600225356RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC55110.43853715908132396RNA PCR Primer, Index 41 (96% over 26bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA44830.35673418329914264No Hit
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG42730.340023458674378Illumina PCR Primer Index 11 (95% over 24bp)
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATG31900.2538438645380917No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31610.25153619304229086No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTCGACATCTC24170.19233248294312466No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT19330.15381824142700037RNA PCR Primer, Index 41 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG15210.12103339121079545Illumina PCR Primer Index 11 (95% over 24bp)
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCTCGACATCTCGT13910.11068865691927446No Hit
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT13860.11029078252344673RNA PCR Primer, Index 41 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74000.087.653381
TCCGTAC45950.079.369372
CGTAGGG2850.079.154042
CCGTACA44350.078.947683
CGTACAC44300.078.394014
ATCCGTA46950.078.300881
GCTACGA8300.077.568374
CGCTACG8350.077.110023
TAGGGCA7300.076.606614
CTACGAC8500.075.743235
ATGCGGG5250.075.1963352
GACCGAT750.075.190358
AGGGATG19900.075.0958945
ATAGCGG1950.074.8304441
GTAGGGC5850.074.714313
TAGGGCG2150.074.316054
TAGAGGG19400.074.130412
GTACACC47450.073.189775
CGAAGGG8950.072.990862
AGGGCAT11800.072.8804865
Produced by FastQC (version 0.11.5)