FastQCFastQC Report
Mon 27 Feb 2023
SRR3128158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387158
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164711.1873917751258327No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGACATC65430.4716838312578668No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG65120.46944904617931055RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC59180.426627680480522RNA PCR Primer, Index 41 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG47950.345670788763789Illumina PCR Primer Index 11 (95% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36150.26060477609616206No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA31740.22881315610766761No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT19820.14288206534511572RNA PCR Primer, Index 41 (95% over 24bp)
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATG17660.12731065963646535No Hit
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT15790.11382985932388379RNA PCR Primer, Index 41 (95% over 24bp)
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTCGACATCTC14360.10352101202602733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84950.087.792261
TAGGGCG1900.086.578814
GACGGGT2100.080.5713043
ATAGCGG1900.079.197741
ACGGGTA1800.078.333214
TAGGGCA8800.077.977164
CGAAGGG11550.076.5020452
CGCTACG7750.075.8063353
AGGGCAT13600.075.680985
AGGGATG24250.075.584795
GTAGGGA10400.073.2114263
GTAGGGC6450.072.868113
AGAGGGC17600.072.1021653
TAGAGGG20500.071.9901352
TCCGCTA8250.071.817951
ATGCGGG5900.071.69482
AAGCGGG14850.071.528512
CGAGGGA7650.071.267873
TAGCGGG6400.071.234272
AGGGTAT8650.071.176525
Produced by FastQC (version 0.11.5)