FastQCFastQC Report
Mon 27 Feb 2023
SRR3128157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1202316
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144671.2032610395270462No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG54290.4515451844606576RNA PCR Primer, Index 41 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC49760.41386790161654674RNA PCR Primer, Index 41 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG40600.337681607830221Illumina PCR Primer Index 11 (95% over 24bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGACATC34820.2896077237598102No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30830.25642177264546095No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT18160.15104182261568505RNA PCR Primer, Index 41 (95% over 24bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA16700.13889859238336677No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG12990.10804147994370865No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG12340.1026352473060327No Hit
AGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT12150.10105496391963512RNA PCR Primer, Index 41 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73750.088.3409651
CGTAGGG2400.086.278562
CGCTACG6050.083.231913
AATGCGG2350.080.1272351
GTAGGGC5800.078.705523
TAGGGCG1750.077.9868554
CGACCAA6400.077.1035468
TAGGGCA7400.076.951144
TCCGCTA6750.076.0170061
AGGGATG21100.075.735175
GCTACGA6650.075.722264
TAGAGGG17850.074.084872
CGAAGGG9550.073.917852
GAGGGTA6900.073.660744
ATAGCGG1800.073.2273941
CTACGAC6950.073.036525
CGAGGGA6600.072.017643
ACGGGTA1250.071.532774
AGGGCAT11450.071.424125
TAGCGGG5350.071.2512742
Produced by FastQC (version 0.11.5)