FastQCFastQC Report
Mon 27 Feb 2023
SRR3128156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1211103
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153161.2646323227669323No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTATCGT82200.6787201418871889No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC53640.44290204879353773No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG49000.40458986560185217No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG42790.35331429283884197No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA41130.3396077790245751No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATG25960.2143500594086548No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCCTATCGTTC19320.15952400415158743No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT15190.12542285833657418No Hit
ACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG14510.1198081418343444No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT13960.11526682701636443No Hit
AGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT12340.10189059064340522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60200.087.1133651
TAGCGGG6350.077.704352
CGTACAC33750.077.276774
CCGTACA34000.076.9849853
ATCCGTA35350.076.371081
ATAGAGG7000.075.925361
TCCGTAC35400.075.798852
CATACGG507.094059E-1175.253451
ACGGGTA1500.075.188214
AAGGGTA8200.074.500514
GTACACC35250.073.321835
CGTAGGG3400.073.253222
AGGGTAC6550.073.179375
TACACCC34000.071.871086
TAGGGTC3050.070.874144
ATAGGGA9750.070.850423
ATAACGG200.002156920570.550111
GTAGGGT5150.070.2608263
ATAGGGC6900.070.1484153
TATAAGG4600.069.527641
Produced by FastQC (version 0.11.5)