FastQCFastQC Report
Mon 27 Feb 2023
SRR3128154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162262
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164161.4124181983064061No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC46390.39913547891955514No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG44960.38683188472134505No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG37580.3233350139641492No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTATCGT25430.21879748283949746No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT14520.1249288026279789No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA14350.12346613758343644No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14060.12097100309568755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60100.087.396261
AGGGTAC5750.082.554295
CGTAGGG2900.079.493732
TAGCGGG4750.077.256632
AGTAGGG18100.072.7803652
TAAGGGA10300.071.712883
TAACGGG3150.071.691142
TAGAGGG17750.071.2998662
TAGTAGG4700.071.099021
ATAAGGG15200.070.8804862
ATAGGGA12000.069.786843
GTAGGGT4550.069.278463
GAGGGTA6250.069.253644
AGGGATC10500.068.931485
GAATAGG6500.068.788111
TAAGGGT4600.068.525433
ACGGGTA1100.068.432454
AGGGATG13250.068.103825
GTAGGGC5600.068.050573
TACGGGT900.067.957223