FastQCFastQC Report
Mon 27 Feb 2023
SRR3128153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338493
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151491.1317952353878578No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTACCAGTA90120.6732945185368919No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC53810.4020192858685104TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG50620.3781865127423154No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA43730.32671071122523615No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG41490.3099754724156196No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATG26180.19559310358739268No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCTACCAGTATC19510.14576094159625788No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT16690.12469247130915141No Hit
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT15520.11595129746662851TruSeq Adapter, Index 15 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG14290.10676185829884804TruSeq Adapter, Index 15 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT13810.10317573569678735TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60450.086.711131
ATCCGTA37500.079.927111
CCGTACA35850.079.7011643
TCCGTAC37400.079.6650542
CGTACAC36150.078.649744
ATTACGG800.076.4608841
GTACACC37300.075.216955
ACGGGTA1000.075.191754
TAGCGGG5000.074.2518542
TAGGGCA8000.074.0168844
TACACCC36350.073.8213356
AGGGCAT12000.072.842015
AGGGATG11050.072.299755
ATAGCGG2300.071.6021651
GTACGGG3300.071.204312
ATAGGGC8550.070.354853
GAATAGG8500.069.748931
AGGGAAT13050.069.501955
AGTAAGG8400.069.458971
AGAGGGC19950.069.023
Produced by FastQC (version 0.11.5)