FastQCFastQC Report
Mon 27 Feb 2023
SRR3128147.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128147.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154794
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266891.2385870760731652No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGTATTCA48860.226750213709524No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA44820.2080013217040701No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC43560.20215389498949785No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG42300.1963064682749256No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG34380.15955121464047145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95500.086.267391
TCCGTAC25850.078.5349042
CCGTACA25400.077.1510543
TAGGGCA10700.076.419654
CGTACAC25450.076.0762254
ATCCGTA27100.075.871031
CGTAGGG4400.072.626612
AGGGATG34400.071.720025
AGGGCAT19100.070.1214755
AGGGTAC11150.069.96375
GTAGGGA16850.069.723583
GAGGGAT30650.069.302284
ATAGGGC12900.069.215513
AAGGGAT34200.068.704224
TAGAGGG35200.067.953942
ATAAGGG29800.067.809692
AGTAAGG14850.067.803361
AGTAGGG36450.067.6863862
ACGGGTA3750.067.670914
TAGGGTA6900.066.744664
Produced by FastQC (version 0.11.5)