FastQCFastQC Report
Mon 27 Feb 2023
SRR3128146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396327
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318471.3289922452152816No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC50200.2094872694753262No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG47420.19788618164382404No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG37510.15653122466174274No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA31490.1314094445374108No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGTATTCA28010.11688721948214914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107150.086.149621
TAGGGCA13700.075.1268164
CGTAGGG6050.074.573982
AGGGATG37250.073.555255
GAGGGAT33300.071.977614
GTACGGG5050.071.6590042
GGTAAGG14100.071.370691
GTAGGGA19200.070.495713
AGTAGGG40100.070.319912
ATAGCGG4550.070.278551
AGGGCAT22650.069.92515
ATAGGGC16400.069.349443
TCCGTAC17050.068.910762
GACCGAT1850.068.588998
GTAGGGC12350.068.497863
AGAGGGC32200.067.576643
AAGGGCA41100.067.465484
CGAAGGG23700.067.422062
AGGGAAT25600.067.374815
TATAGGG20300.066.907262
Produced by FastQC (version 0.11.5)