FastQCFastQC Report
Mon 27 Feb 2023
SRR3128145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098621
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275921.3147681263077038No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC39120.18640812228601544No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG38260.1823101932173556No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG31800.15152807486439904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99450.086.685381
TAGGGCA11200.076.9042364
AGGGATG35200.073.303645
CGTAGGG6350.072.6389542
GGTAAGG12000.072.1936341
AGGGCAT18450.072.091815
GAGGGAT30750.071.633794
TAGCGGG9350.070.474762
TAGGGTA8650.070.192514
CGAAGGG19900.069.77282
GTAGGGA17300.069.6483843
GTACGGG5050.068.9695742
AAGGGAT34600.068.968234
ATAGGGC13600.068.87023
TAGAGGG34700.067.9556352
AGAGGGC28700.067.894663
AAGAGGG73150.067.8177642
AGGGATC18100.067.5135045
AAGGGAC20800.067.432634
ATAGCGG4050.067.427231
Produced by FastQC (version 0.11.5)