FastQCFastQC Report
Mon 27 Feb 2023
SRR3128142.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128142.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445119
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205101.4192602823712095No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG39050.2702199611243088No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC37160.25714145340279937No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG34280.23721229877954686No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAGCTCCA25370.1755564766638595No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20970.14510915710055713No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC19590.13555977051024864No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA15330.10608122929668769No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT74550.087.0512851
GTACGGG2650.083.451722
TACGGGT950.079.246433
TAGGGTA5300.076.349454
ATAGCGG3350.075.859071
ACGGGTC1250.075.284114
AGTAGGG21650.075.1971742
TAAGGGA12550.074.234333
AGTACGG1650.074.156281
CGTAGGG4650.073.867472
TAGGGAC8200.072.87414
TGTAGGG8600.071.6730962
AGGGCAT11050.071.46025
AGGGATG21250.071.4431845
ACGGGAT5150.071.2640844
AGGGTAT7800.071.105735
ATAGGGA13900.070.409593
TAGCGGG7250.070.0920942
TAGGGCA8000.069.990694
GTAGGGA11200.069.7386253