FastQCFastQC Report
Mon 27 Feb 2023
SRR3128141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252860
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158521.265265073511805No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTACGCA69350.5535335153169548No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG47080.37578021486838115No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC44980.35901856552208544No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA37990.3032262184122727No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG37920.3026674967673962No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21880.17464042271283306No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATG20070.16019347732388295No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCCTACGCATC16450.13129958654598278No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT15370.12267930973931644No Hit
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG13660.10903053812876139No Hit
AGAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT13450.10735437319413182No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT13120.10472039972542822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT65300.087.10941
AGGGATG21300.079.875325
ATAGCGG1800.078.4152761
CCGTACA30850.077.391313
CGTACAC30800.077.0591664
GGATGGC19250.075.9299857
CGTAGGG2850.075.854142
CGAAGGG12300.075.6530462
GGTAAGG6750.075.278661
TCCGTAC33000.075.194542
ATCCGTA33200.074.966891
GAGGGAT19050.074.013324
AGGGCAT10200.073.7230765
TACGGGT1350.073.108733
TAGGGTA3350.072.952844
TAAGCGG3300.072.712351
ATGGCAC18050.072.3854459
AAGGGAT22100.072.092674
AGTAAGG6550.071.830791
TAGGGCA7700.071.4132464
Produced by FastQC (version 0.11.5)