FastQCFastQC Report
Mon 27 Feb 2023
SRR3128140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386793
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194151.3999926449008613No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG51050.36811550101565266No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC46170.33292639925352957No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG42150.30393865558882976No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTACGCA40240.29016587190734305No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA26810.1933237332464182No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC25630.18481489306623267No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT16490.11890743607733814No Hit
AGAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT14880.10729791684844098No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT14160.1021060821622261No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76000.087.750641
ATAACGG1050.080.625211
TAGCGGG6400.077.1163252
CGTAGGG2700.076.59952
AGGGCAT13200.076.198345
AGGGATG25600.075.641995
AGTAGGG19850.075.0647052
GTACGGG3350.074.364912
TAAGGGA12100.073.031383
AAGGGAT27050.072.977294
TAGGGCA9800.071.940084
GGTAAGG6650.071.4311141
TAGGGTA3900.071.103844
AGAGGGC16600.071.072673
TAGAGGG22650.070.973282
GGATGGC20200.070.966637
AGGGTAC5900.070.899585
AAGGGTA7700.070.806474
AGTACGG1800.070.547061
CGAAGGG12350.070.41122