FastQCFastQC Report
Mon 27 Feb 2023
SRR3128139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191589
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169941.426162879986304No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG42920.36019130757333273No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC40520.3400501347360541No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG35220.29557171138706384No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20290.17027683202849303No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCTACGCA19700.16532546037266205No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT14530.12193801721902434No Hit
AGAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT13260.11127997992596439No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA13030.1093497841957252No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT12620.10590900050269011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64050.086.9411851
ACGGGTA1200.082.358174
AGGGATG20200.078.4085855
TAGGGCA6950.077.1949164
TAGCGGG5200.076.9279562
AAGGGCG4650.076.918234
AGGGCAT10650.076.7864765
CGAAGGG10100.075.485282
GTACGGG3000.075.29892
AATGCGG1950.074.831931
TAGAGGG19450.074.766582
CGTAGGG1900.074.308132
AAGGGCA16500.073.017124
GAGGGAT19550.072.93984
TAGGGTA3100.072.86994
AAGGGAT22800.072.656844
ATAACGG1300.072.4181
ATAGCGG1700.071.992011
AGAGGGA30300.071.7576143
TAGACGG1850.071.243661