FastQCFastQC Report
Mon 27 Feb 2023
SRR3128137.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128137.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178681
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319861.4681359960453138No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC74280.3409402294323951No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG64900.297886657110426No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG59830.2746156963777625No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCTGTAT46220.212146707113157No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA37650.172810980588714No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26690.12250531399502727No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT22130.10157521913488023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107550.082.757761
AGGGTAC13350.079.567595
TAGGGTA10200.074.188174
TAAGGGA16400.072.794553
GTAGGGT10750.071.704163
ATAGCGG4600.071.549841
ATAGGGC11100.071.1369553
ATAGGGT8650.070.0942843
GTAGGGA15050.069.955283
CGTAGGG5250.069.830372
GATTAGG5050.069.829411
GGGTACC15700.069.753386
AAGGGTA16450.069.144634
GAGGGTA12400.068.985644
ATAGGGA19150.068.722393
TAGCGGG9000.068.4128652
ATGCGGG12300.068.400132
AATGCGG5300.068.309851
AGTAGGG33400.068.1095352
TAGGGTC5800.068.070714