FastQCFastQC Report
Mon 27 Feb 2023
SRR3128136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910936
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269961.412710839086186No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC57190.29927742216379827No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG52670.2756240920679709No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG49150.2572037996039637No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCTGTAT27230.14249561471446454No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT23790.12449396526100298No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA19850.10387579699163133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91400.084.055751
TAGGGTA8650.077.799514
AGGGTAC11800.077.277575
ATAGGGC9400.074.595963
GTAGGGT9950.073.310393
AGTAGGG27700.072.3746952
TAGGGCA7700.071.5075764
AAGGGTA14750.071.468214
TAGGGCG3350.070.239614
ATAGCGG3900.070.0094761
GTAGGGA13000.069.504793
GAATAGG12500.069.294891
AGGGTAA10450.069.268895
ATAGGGT6800.069.206673
AAGGGTC12450.068.795334
ATAGGGA17400.068.156643
CGTAGGG5050.068.028112
AGTAAGG10750.067.876071
TAAGGGA14300.067.79353
ATAGAGG11100.067.432241
Produced by FastQC (version 0.11.5)