FastQCFastQC Report
Mon 27 Feb 2023
SRR3128135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1937526
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253781.3098146811965363No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCGCTAAAT69360.3579822928827794No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC51960.2681770463983451No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG42940.22162283241618433No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG42750.22064220041434282No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA42720.2204873637824731No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28630.14776575901433064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81400.085.075511
CCGTACA30600.075.101533
ATAAGGG22300.075.0250852
CGTACAC30400.074.977254
TAGGGTA8750.074.6565864
TCCGTAC31700.073.978012
AGTAGGG32500.073.603052
GTAGGGT9650.073.050863
AGTAAGG13050.072.82191
ATAGGGA15050.072.758013
AGGGCAT16400.072.55
TAGGGCA9100.072.301624
ATCCGTA32750.072.256651
GAATAGG11150.072.1511
AGGGTAC11750.071.993955
ACGGGTA2250.071.016264
AGGGATG24100.070.591585
AGATAGG9150.070.44021
ATAGAGG12100.070.374341
ACTAGGG8300.070.210972
Produced by FastQC (version 0.11.5)