FastQCFastQC Report
Mon 27 Feb 2023
SRR3128134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143023
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308771.4408151475742446No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC57280.26728597873191284No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG48300.2253825553902128No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG46190.21553665079656167No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCGCTAAAT40330.18819210059808036No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT31700.14792188417949784No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA29200.13625612044294438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92850.086.860031
AGTAGGG37300.073.969762
AGTAAGG13000.073.071131
GTAGGGT11400.072.978233
GAGGGAT21350.072.8693544
ATAGCGG4500.072.106491
TAGGGTA11100.071.9847954
ATAGAGG11900.071.922071
TAGGGCA9800.070.982364
GGTAAGG12000.070.930841
AGGGTAA14950.070.109765
AGGGCAT18100.069.334195
GATTAGG5400.068.797261
AAGGGTA19050.068.590594
GAATAGG11400.068.476421
AGACGGG15100.068.169812
AGATAGG11600.067.701191
ACTAGGG10000.067.684222
AGGGATG25650.067.616675
AGAGGGC29450.067.511853
Produced by FastQC (version 0.11.5)