FastQCFastQC Report
Mon 27 Feb 2023
SRR3128133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868188
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267071.4295670457148852No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC46210.24735197956522575No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG38630.20677790457919654No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTG37270.19949812331521238No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT26760.14324040192957027No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCGCTAAAT21030.11256897057469592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84850.086.715151
GAATAGG10300.074.49691
TAGGGCA7850.073.123484
AGGGTAC13400.072.9558265
GAGGGAT19500.072.6270754
ATAGGGA15450.072.479493
AGGGTAA11250.072.2761465
AGTAGGG32250.071.633872
AGTAAGG12550.071.268521
AGGGATG24150.070.841175
TAGGGTA9850.070.695564
TAGTAGG7100.070.280581
AGGGCAT16700.070.078425
AGAGGGC26250.069.545513
GGTAAGG10850.069.418961
AGACGGG11650.069.061642
AGATAGG9300.068.334281
TAGAGGG32050.068.2639852
ATGAGGG30300.068.169262
CGTAGGG5350.067.7179262
Produced by FastQC (version 0.11.5)