FastQCFastQC Report
Mon 27 Feb 2023
SRR3128132.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128132.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131519
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150851.3331636499254542No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCGTTGA92090.813861720395327No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC42770.37798746640577846No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG41560.36729387663839497No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG31860.2815684049494529No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA28180.24904575177261715No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATG28100.24833873757312075No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGTCGTTGATC21920.19372189066202158No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT14700.12991385915746886No Hit
ACTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG12600.11135473642068759No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT12030.10631726024927554No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC11840.10463810152547151No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGT11530.10189842150242286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64700.082.6857451
CGTAGGG3000.081.462422
TAGCGGG5700.080.80282
CCGTACA35400.075.806783
ATAGCGG2550.075.6515961
GAATAGG6850.075.557321
CGTACAC35350.075.38224
TCCGTAC37300.074.465282
ATCCGTA37350.074.325161
AGGGTAC4500.073.10735
TAAGGGA9250.071.63953
TACACCC35150.071.3988346
AGGGCAT9600.070.496325
AAGGGTA7000.070.4963154
TCCGCTA2900.069.766171
AATAGGG15750.069.526532
GTACACC38300.069.453295
AGTAGGG15300.069.421222
TAGGGCA4700.068.99644
ATGAGGG15750.068.631342
Produced by FastQC (version 0.11.5)