FastQCFastQC Report
Mon 27 Feb 2023
SRR3128131.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128131.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256723
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184331.4667512252103287No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCGTTGA55920.4449667906133651No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC45070.3586311382858434No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG44770.3562439773919949No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG34270.27269334610729656No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA20440.1626452289008795No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATG16350.13010026871474462No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT14930.1188010404838616No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14730.11720959988796259No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC13840.11012768923621195No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGTCGTTGATC13350.10622865977625937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75500.084.463291
TAGCGGG6600.080.4661562
CGTAGGG2850.077.505362
ATAGCGG2700.076.638011
GTAGGGT4200.076.091893
GGTAAGG6150.074.1740341
ATAGGGC7400.073.672443
TAGGGTA4950.072.158454
AGGGCAT10200.071.879195
ACGGGTA1250.071.436854
AGGGTAC5150.071.181335
AGTAGGG18700.070.873882
AAGGGTC8350.070.3561864
TAGGGCG2450.069.058184
CCGTACA24000.068.7344743
AGGGTAA5900.068.5054555
CGTACAC24250.068.413484
AGGGAAT13400.068.392515
TCCGTAC24750.068.170742
GAATAGG7150.067.746441
Produced by FastQC (version 0.11.5)