FastQCFastQC Report
Mon 27 Feb 2023
SRR3128130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062832
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157791.4846184533397564No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG38560.3628042813916028No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC38560.3628042813916028No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG29350.27614900567540307No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTCGTTGA28790.27088006382946694No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT13170.12391422162674814No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT12580.11836301503906543No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG10940.10293254249025247No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC10920.10274436599575475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66500.084.798981
ATAGCGG2150.081.073971
CGTAGGG3200.075.047042
TAGCGGG5050.074.595392
TAGAGGG14800.073.1778262
TACACGG1100.072.8072051
AGGGTAC4400.072.646185
TAGGGAC4700.072.131934
AAGGGTA7050.072.131924
GGTAAGG6300.071.78751
AGGGCAT9250.070.636575
GTAGGGA9000.069.582823
AGTAGGG15950.068.4921342
GAATAGG6000.068.310291
TAAGGGA7450.068.259073
AATAGGG14950.068.033982
GTAAGGG12800.066.953732
AGGGATG12500.066.937055
ACGGGAT3100.066.831914
ACGAGGG8900.066.664392