FastQCFastQC Report
Mon 27 Feb 2023
SRR3128129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923309
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238471.2398943695474829No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTGACGTGC65140.33868712723748495No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA37910.19710821298085748No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC35710.18566959339346928Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG33710.17527084831402545TruSeq Adapter, Index 22 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG30180.15691706324880714TruSeq Adapter, Index 22 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85850.084.66611
CCGTACA26150.079.43653
TCCGTAC28400.076.452752
CGTACAC27150.075.818254
ATCCGTA28850.075.6647341
ATAGGGC11600.074.546863
TAGGGTA7250.073.898634
TAGGGCA9800.072.89324
CGTAGGG5300.072.7122342
AGGGTAC10550.072.611375
GGTAAGG11700.071.1719361
AGATAGG10600.070.568791
ATAGAGG11850.070.271031
ACGGGTA2750.070.068154
ATGCGGG8350.069.7918552
AGTAGGG29550.068.3880842
TAGACGG2550.068.262611
AGGGATG25350.067.853565
ATAGGGA15950.067.7698753
GTACACC30350.067.359695
Produced by FastQC (version 0.11.5)